| 摘要 |
The process is characterised according to the invention in that: 1 a liquid phase is prepared consisting essentially of a solution of the protein and optionally of a biologically active substance in water or in an aqueous mixture at a temperature below the coagulation temperature of the protein, and to which one or more surfactants may be added, 2 a second liquid phase is prepared, consisting essentially of water or of an aqueous mixture at a temperature above the coagulation temperature of the protein, which may contain a biologically active substance and to which one or more surfactants may be added, 3 one of the liquid phases obtained under 1 or 2 is added with moderate stirring to the other under pH conditions far from the isoelectric point of the protein, so as to obtain practically instantaneously a colloidal suspension of nanoparticles of the protein and optionally of the biologically active substance, and 4, if desired, all or part of the water or of the aqueous mixture is removed, so as to obtain a colloidal suspension of desired concentration of nanoparticles, or to obtain a nanoparticle powder. Applications: biochemistry, pharmacy, medicine, cosmetics.
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