发明名称 |
POSITIVE SELECTION SYSTEM FOR STABILISATION OF EXPRESSION VECTORS |
摘要 |
The invention relates to a method for the construction of plasmids assuring the insertion of a nucleotide sequence into a chromosome and the simple separation of clones containing the transformed chromosome by using methods of genetic engineering known per se which comprises inserting a copy of a chromosomal segment corresponding to a chromosomal segment of the host cell between the transcription regulating sequences capable of expressing foreign gene into a plasmid containing transcription regulating sequences capable of expressing foreign gene and a resistance gene (R2) expressed independently from the said regulating sequences, then downstream inserting a resistance gene without own promoter (R1) different from (R2), and finally inserting a sequence containing at least one transcription terminator into the inserted copy of the chromosomal segment. |
申请公布号 |
WO9000608(A1) |
申请公布日期 |
1990.01.25 |
申请号 |
WO1989HU00036 |
申请日期 |
1989.07.11 |
申请人 |
RICHTER GEDEON VEGYESZETI GYAR R.T. |
发明人 |
LUKACSOVICH, TAMAS;VENETIANER, PAL;BALIKO, GABRIELLA;GAAL, TAMAS |
分类号 |
C12N15/20;C12N15/68;C12N15/90;(IPC1-7):C12N15/20 |
主分类号 |
C12N15/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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