摘要 |
PURPOSE:To obtain alkaliphilic cellulase in good productivity by cultivating a bacterium capable of producing alkali cellulase in a medium having an iron- containing substance. CONSTITUTION:A bacterium [e.g., Bacillus sp. KSM-635 (FERM P-8872)] capable of producing alkali cellulase is inoculated into a bacterium comprising >=0.1ppm calculated as iron of iron-containing substance (e.g., FeSO4), 0.1-2% nonionic surfactant, carbon source (e.g., CMC), nitrogen source or organic nutrient source (e.g., peptone) and an inorganic salt (e.g., Na2CO3) at pH7-11 and cultivated at 25-37 deg.C for half a day-5 days. |