| 摘要 |
<p>A method of producing recombinant human Protein C, the method including providing a vector including a DNA sequence encoding human Protein C under the transcriptional control of an eukaryotic metallothionein promoter, the vector further including at least the 69 % transforming region of the bovine papilloma virus; transfecting host eukaryotic cells with the vector; adhering the transfected cells to carrier particles; culturing the carrier particle-bound cells in culture medium to produce the recombinant Protein C; and isolating the recombinant Protein C from the culture medium or the cells. The Protein C is ideally activated by contacting same with thrombin bound to Sepharose beads in low salt concentration. Protein C produced by C127 cells following the above method are unique in that they possess an α-1-3 galactose linkage embueing the protein with longer in vivo half-life.</p> |
