Generation of chimeric antibodies in vivo by site-specific recombination.

摘要

An in vivo method of maxing chimeric antibodies is disclosed. The method comprises the steps of: a) construction of a transfection plasmid which comprises, in operable association with DNA control regions and selectable markers, a gene segment comprising a gene or gene segment for an immunoglobulin constant heavy or light chain and a gene or gene segment for another function; b) transfection of the plasmid into a recipient mammalian antibody producing cell that secretes an immunoglobulin having at least seventy (70) percent DNA sequence homology with the immunoglobulin gene or gene segment in the plasmid; c) screening for the stably transfected antibody producing cells that secrete the chimeric antibody. h