| 摘要 |
A process for synthesizing an oligonucleotide comprising linking a nucleoside phosphate to a solid support, through the heterocyclic moiety of the nucleoside, coupling a mono- or oligonucleotide to the nucleoside phosphate through its phosphate moiety, in at least one step enzymatically lengthening the mono- or oligonucleotide, cleaving the resultant oligonucleotide from the solid support-nucleoside phosphate at the phosphate moiety of the nucleoside, and separating the oligonucleotide. After cleaving and separating the solid support-nucleoside phosphate is recycled for further coupling. Advantageously the solid support-nucleoside phosphate is phosphorylated between separation and recycling. |
