| 摘要 |
<p>Conjugate for enzyme immuno determinations The present invention provides a process for the preparation of a conjugate, suitable for enzyme immuno determinations, from a labelling enzyme, which contains a carbohydrate part, such as peroxidase, and an immunologically effective substance with the use of a coupling method acting on the protein part of the labelling enzyme, wherein, before or after coupling with the immunologically effective substance, the labelling enzyme is oxidised with periodic acid or with an alkali metal salt thereof in an aqueous medium and the oxidation product is then reduced with sodium borohydride. The present invention also provides a conjugate which can be prepared by this process and a reagent containing it.</p> |
