发明名称 |
Time-resolved fluorimetric detection of lanthanide labeled nucleotides. |
摘要 |
<p>Nucleotides conjugated with a chelating agent and labeled with a lanthanide, especially terbium, are readily detected by time-resolved fluorimetry during and following gel electrophoresis. The labeled nucleotide conjugates are very stable, highly fluorescent with a long radiative lifetime and remain fluorescent during dilution and during gel electrophoresis and since an enhancement solution is not required for detection, the labeled nucleotide conjugates can be detectd on-line, if desired.</p> |
申请公布号 |
EP0340675(A2) |
申请公布日期 |
1989.11.08 |
申请号 |
EP19890107782 |
申请日期 |
1989.04.28 |
申请人 |
THE PERKIN-ELMER CORPORATION |
发明人 |
PICOZZA, ENRICO G.;SAAVEDRA, STEVEN S. |
分类号 |
G01N21/64;C12Q1/68;G01N21/78;G01N27/447;G01N33/533;G01N33/58 |
主分类号 |
G01N21/64 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|