| 摘要 |
<p>A vaccine for immunizing animals against diseases caused by microorganisms producing an osteolytic toxin comprising an immunologically effective amount of a recombinant, immunogenic, detoxified Pasteurella multocida toxin or toxin analogue, the vaccine being encoded by a nucleotide sequence coding for a Pasteurella multocida toxin or toxin analogue which is inserted in an expression vector capable of replicating in a suitable host microorganism in which the said sequence may be expressed. Optionally, the produced toxin or toxin analogue is subjected to posttranscriptional modifications to provide the detoxified toxin or toxin analogue. Alternatively, the nucleotide sequence may be modified prior to insertion into the expression vector so as to provide the toxin or toxin analogue in a detoxified form. Furthermore, the use of a monoclonal antibody against Pasteurella multocida toxin or toxin analogue, a labelled DNA sequence homologous to a DNA sequence coding for a toxin or toxin analogue, and the toxin or toxin analogue per se as diagnostic agents are described.</p> |
