| 摘要 |
PURPOSE:To obtain an antibody, consisting of immunoglobulin prepared from eggs produced by a hen immunized with a water-insoluble glucan synthetase produced by Stretococcus.mutans, capable of inhibiting sticking of the Streptococcus.mutans to tooth surfaces and useful as an active ingredient of an anticarious agent. CONSTITUTION:An antibody prepared by culturing Streptococcus.mutans having a serotype of a, d or g as a cariogenic pathogenic germ in a culture medium containing at least glucose to provide a water-insoluble glucan synthetase (TGF-1) from the culture supernatant thereof, immunizing a hen (preferably egg breed, such as White Leghorn) using the above-mentioned enzyme as an antigen, collecting eggs from the hen after the passage of >=1 months from the immunization and preparing the antibody having inhibitory activity against the TGF-1 therefrom. An IgG fraction can be specifically separated and purified by simple operation, such as migration of only the IgG fraction in immunoglobulin into egg yolk and preparation of the antibody from the egg yolk and a high antibody value can be normally obtained for about 3 months. |
