发明名称 |
MICROBIOLOGICAL TEST PROCESSES AND APPARATUS |
摘要 |
An improvement in the method of carrying out a microbial culture test, in which the culture medium of a microorganism under test includes a hydrolysable fluorogenic substrate which is enzymically hydrolysable to yield a fluorescent material, and in which test the growth is assessed by fluorescence, wherein said improvement comprises inoculating the microorganism into the culture medium at an inoculum concentration within the range 5 x 10<4> organisms/ml to 10<6> organisms/ml; including one or a plurality of hydrolysable fluorogenic substrates in the culture medium with the concentration of each fluorogenic substrate in the medium in the range 30 micromolar to 300 micromolar; and after cultivating the inoculated medium by incubation, measuring the fluorescence yield of the culture and retaining the culture in a form suitable for possible further incubation to yield a confirmatory culture result by another method of estimation. |
申请公布号 |
DE3380263(D1) |
申请公布日期 |
1989.08.31 |
申请号 |
DE19833380263 |
申请日期 |
1983.04.14 |
申请人 |
RADIOMETER CORPORATE DEVELOPMENT LIMITED |
发明人 |
CARR, ANTHONY HUGH;BADLEY, ROBERT ANDREW;JOBLING, IAN;SANDS, THOMAS JOHN |
分类号 |
G01N33/52;C12M1/34;C12Q1/04;C12Q1/06;C12Q1/18;G01N21/76;(IPC1-7):C12Q1/04 |
主分类号 |
G01N33/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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