发明名称 |
Method for purification of HBc antigen and method for measurement of HBc antibody by using said purified HBc antigen |
摘要 |
A method for the purification of HBc antigen, particularly HBc antigen produced by recombinant organisms by means of DNA recombination technique, which comprises subjecting a solution containing HBc antigen to acid-treatment, wherein the solution containing HBc antigen is acidified to a pH range of not higher than 6 by adding an acid and then resulting precipitates of lipid and contaminant proteins are removed, and then subjecting the acid-treated solution containing HBc antigen to an ion exchange chromatography with an anion exchanger, and a method for measuring HBc antibody by using said purified HBc antigen in a passive hemagglutination method, EIA method or RIA method.
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申请公布号 |
US4839277(A) |
申请公布日期 |
1989.06.13 |
申请号 |
US19850811399 |
申请日期 |
1985.12.20 |
申请人 |
JURIDICAL FOUNDATION THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE |
发明人 |
SUGAHARA, KEISHIN;IMAMURA, TAKAYUKI;HAMADA, FUKUSABURO;OHTOMO, NOBUYA;FUJITA, HARUO;YAGAMI, KAZUHIDE |
分类号 |
C07K14/02;G01N33/576 |
主分类号 |
C07K14/02 |
代理机构 |
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代理人 |
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主权项 |
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