Plasmids, methods for their construction, microorganisms carrying them and methods for the extracellular production of xylanase by cultivation of the microorganisms.

摘要

Novel plasmids, methods for their construction, transformed microorganisms containing them and methods for the extracellular production of xylanase by cultivation of the transformed microorgansims are described. One of the microorganisms is escherichia coli HB101 carrying plasmid pCX311 which is capable of extracellularly producing xylanase. The methods for the cultivation of the microorganisms are characterized by using a medium containing NaCl (or KCl), or NaCl (or KCl) and bran, or NaCl (or KCl) and xylan and carrying out the culturing for 12 to 48 hours.