| 摘要 |
<p>A method for removing endotoxin contaminants from a biological material is provided. A biological material such as a protein preparation to be decontaminated is contacted with a detergent which solubilizes and removes endotoxin but which does not denature the active biologic component. The detergent can be a bile acid such as taurodeoxycholate or a bile acid/N-alkylsulfobetaine such as CHAPS. A chelating agent can be used with the detergent to remove divalent cations. The endotoxin content of protein preparations can be reduced to less than 0.1 EU/mg biological material by this method. The method is particularly applicable to the purification of immunoglobulins especially those that have a binding affinity for endotoxin such as anti-endotoxin antibody.</p> |
