Human immunoglobulines produced by recombinant DNA techniques.

摘要

Recombinant human immunoglobulins and methods for their production are provided. DNA constructs containing operably linked genes coding for human light and heavy chains are transfected into eucaryotic host cells capable of expressing the human immunoglobulin sequences. Using these methods the isotype of an immunoglobulin may be switched, or a monoclonal antibody may be produced in a new host cell free of a transforming virus' genome. Also disclosed is the use of cytomegalovirus transcriptional enhancer sequences to increase expression of immunoglobulin genes. The recombinant immunoglobulins find use in a number of ways, including prophylaxis and therapy, diagnosis, and as immunogens.