摘要 |
A vector which contains at least a first consensus sequence which is, in the first three nucleotides and at least in a total of 10 nucleotides, homologous with the sequence… CTC@ GAGACCAA… and at least a second consensus sequence which is, either in nucleotide 6, 8, 9 and 10 and in at least 9 nucleotides, homologous with the sequence… TTTA@ ATTTTC, or… in at least 10 nucleotides homologous with the sequence… TATGATAATGAG,… or homologous with the sequence… TGG(N)6-7GCCAA… and contains an inefficient selection system, is prepared as described. …<??>Vectors of this type can be amplified quickly, can be transferred universally into mammalian cells and are free of viral origin sequences. |