| 摘要 |
FIELD: biochemistry.SUBSTANCE: invention discloses a method for obtaining a polypeptide and a method of obtaining a reduced amount of G(0) glycoform and/or increased amount of G(1) glycoform of polypeptide. Method for producing polypeptide involves cultivating a cell comprising a nucleic acid encoding polypeptide in a cultivation vessel and recovering polypeptide from culture medium or from cells. Method of producing a reduced amount of G(0) glycoform and/or increased amount of G(1) glycoform of polypeptide involves cultivating a cell containing polypeptide coding nucleic acid in a cultivation vessel and recovering polypeptide from culture medium or from cells. Acidic feed solution and an alkaline feed solution are mixed added to culture medium.EFFECT: invention enables to maintain viability of cultivated cells for a long period of time, reducing time during which nutrient compound is held at pH which reduces stability thereof, use of nutrient solution of solid substances, dispersion and hard-to-mix compounds/solutions, as well as reducing concentration of host cell protein in cell culture supernatant.12 cl, 20 dwg, 3 tbl, 3 ex |
