Simultaneous assay for calcium and phosphorus.

摘要

A method for the simultaneous determination of calcium and phosphorus (inorganic phosphate) substrates with a single reagent system. A reagent system comprising an azo dye in a concentration sufficient to allow an endpoint determination of calcium; and an acidic, aqueous solution comprising a molybdate salt and detergent in a concentration sufficient to allow an endpoint determination of phosphorus. The reagent system is reacted with the specimen such that each of the substrates react with their respective reactant simultaneously. The change in absorbance or fluorescence of the resulting reaction mixture is measured at a plurality of wavelengths which are characteristic for each of the substrates to be determined.