| 摘要 |
PURPOSE:To efficiently produce a lipase, by cloning 1,3-specific lipase gene of a mold, inserting the gene into a pUC based plasmid having a lactose promoter and introducing the obtained plasmid into Escherichia coil. CONSTITUTION:A cDNA bank is prepared from an mRNA of Rhizopus.niveus and an oligonucleotide partially corresponding to the amino acid sequence of a lipase of purified mold is used as a probe to carry out plaque hybridization and afford a hybridizable clone lambdaRL106B. A DNA fragment containing cDNA of the above-mentioned lambdaRL106B inserted thereinto is then inserted into an ECoRI part of pUC8 to provide a plasmid pRL106B, which is subsequently introduced into Escherichia coli JM109. The resultant transformant strain is then cultivated in a culture medium to produce the aimed lipase. |
