| 摘要 |
PURPOSE:To obtain bradykinin having hypotensive action, by separating bacterial cell from E.coli C600 strain containing pBLAK1, treating the cell with cyanogen bromide and separating the objective compound by high-performance liquid chromatography. CONSTITUTION:E.coli C600 strain containing recombinant plasmid pBLAK1 is cultured in a medium, the bacterial cells are collected after cultivation and centrifuged to obtain a protein composed of dihydrofolic acid reductase-reductase fused to bradykinin. The protein is mixed and treated with cyanogen bromide and subjected to high-performance liquid chromatography to obtain bradykinin useful in the fields of medical and pharmaceutical industries, etc. |
