IMMUNOASSAY FOR THE DETERMINATION OF ANTIBODIES AGAINST NATIVE DNA

摘要

1. A method for fluoroimmunoassay of antinuclear antibodies against native DNA characterized in that a. an antigen reagent is prepared by applying substantially pure network DNA comprising kinetoplasts to a solid substrate, b. complexing the antigen reagent in a serum solution derived from a host which produces antibodies to native DNA, c. incubating the complexed antigen reagent in a solution of leaded receptors capable of recognizing and binding these antibodies, and d. evidencing the presence of a labeled receptor bound to the antigen-antibody complex.