发明名称 |
ENZYMATIC POLY-REACTANT CHANNELING BINDING ASSAY |
摘要 |
<p>Improved sensitive immunoassays are provided involving channeling involving one, usually two enzymes, where the enzymes are related by the product of one enzyme being the substrate of the other enzyme. A dispersed aggregation is formed in the assay medium of (1) the analyte, (2) one of the enzymes bound to a second binding member ("SBM") (enzyme - SBM conjugate) which conjugate is non-covalently bound to a first binding member ("FBM"), and (3) a multiplied amount of the other enzyme bound in the complex. The large amount of enzyme or reactant in the complex is achieved by having a multiplicity of linkages binding the enzyme or reactant directly or indirectly to FBMs. The enzyme channeling provides for a detectable signal which can be related to the amount of analyte in the medium.</p> |
申请公布号 |
EP0122059(B1) |
申请公布日期 |
1989.02.22 |
申请号 |
EP19840301676 |
申请日期 |
1984.03.13 |
申请人 |
SYVA COMPANY |
发明人 |
DINELLO, ROBERT;GIBBONS, IAN;ULLMAN, EDWIN FISHER |
分类号 |
G01N33/543;C12Q1/00;C12Q1/54;G01N33/535;G01N33/536;G01N33/542;G01N33/58;(IPC1-7):G01N33/542;G01N33/53 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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