摘要 |
The use of muramoylpentapeptide carboxypeptides for enzymatic synthesis of peptides and peptide derivatives is new. Coupling takes place in water or organic solvent, at (apparent) pH between 4 and 11 and in temp. range 0-70 deg.C, according to the scheme: R1-A-R2 + H-B-R3 -- = R1-A-B-R3 + H-R2. - Where R1 is H or a protecting group e.g. acetyl, A is an amino acid or derivative with D-configuration, R2 is OX where X is alkyl or other gp., B is an amino acid or derivative with D-configuration and R3 is OH, NK2, or OX (where X is e.g. Alkyl). - Further use of aqueous buffer or blends with organic solvents inc. methanol, ethanol, DMF, DMSO are particularly claimed. The use of mumoylpentapeptide carboxypeptidase isolated from bacteria is also claimed. The enzyme may be used in free or immobilised form. |