| 摘要 |
PURPOSE:To provide a DNA fragment containing a base sequence coding a non-A, non-B hepatitis specific antigen protein and useful for the mass- production of a non-A, non-B hepatitis specific antigen protein by a recombinant DNA technique. CONSTITUTION:A liver tissue of a human or chimpanzee affected with non-A, non-B hepatitis is homogenized in an aqueous solution of guanidium thiocyanate and whole RNA is separated as a precipitate by an equilibrium density gradient ultracentrifugation using cesium chloride. The separated whole RNA is purified by the extraction with phenol and the precipitation with ethanol. The RNA is further purified by oligo(dT)-cellulose column chromatography to separate a poly(A)-containing RNA, which is used as a raw material for mRNA. The objective DNA is determined from the mRNA through a cDNA library. The DNA is composed of a sequence of 1,333 bases. |
