摘要 |
<p>The present invention relates to a solid phase competitive immunoassay method for detecting analytes, comprising : (a) coating a surface with antibodies against the analyte to be determined; (b) contacting the coated surface with an aqueous sample containing the analyte to be analyzed, and a conjugate of the analyte with a carrier so as to effect binding between (i) the antibodies and the analyte, and (ii) the antibodies and the analyte-carrier conjugate; (c) removing the solution containing antibody-analyte and antibody-conjugate complexes; and (d) measuring the amount of analyte-carrier conjugate remaining in the solution of step (c) to indicate the amount of the analyte in the sample. e</p> |