| 摘要 |
PURPOSE:To construct a plasmid having bialaphos resistance, by forming a bialaphos-resistant gene to code an enzyme to acetylize dimethylphosphinothricin and phosphinothricin. CONSTITUTION:Total DNA is extracted from a bacterium such as Streptomyces viridochromogenes or a variant thereof capable of producing bialaphos (BA). The DNA is cleft with restriction enzyme Kpn1 to give the aimed gene- containing DNA fragment. The restriction endonuclease cleavage map is as shown by the figure. Then the fragment as a vector is integrated into a proper virus or cyclic plasmid to give a hybrid plasmid. The plasmid having the BA- resistant marker is usable as a vector for recombinant DNA experiment using a ray fungus, a fungus capable of producing a useful substance, especially antibiotics, as a host. |
