NOVEL RECOMBINED PLASMID PGIF1

摘要

PURPOSE:To readily give a fused protein measurable enzymatic activity by making up a novel recombined plasmid pGIF1 including a gene coding fused protein from dehydrofolate reductase and somastatin to solubilize the fused protein. CONSTITUTION:The recombinant plasmid pGIF1 is stably copied in E. coli and gives the host E. coli, resistance to trimethoprim and ampicillin. In the plasmid, the gene giving trimethoprim resistance is recombined by modifying a part of the 3'-terminal of dihydrofolate reductase in Bacillus subtilis to code the fused protein with 4789 pairs of bases. The recombinant plasmid pGIF1 is introduced into E. coli C600 strain and the transformed strain is deposited as FERM P-9301.