发明名称 |
STATIC CELL CULTURE MAINTENANCE SYSTEM |
摘要 |
In apparatus for maintaining animal cells in vitro in a substantially arrested state of proliferation with continuous secretion of cell product, the cells are retained within a reactor vesel chamber (39) in a semi-rigid matrix having interstices for passage of fluid nutrient medium. Fresh nutrient medium is supplied by perfusion into the matrix through relatively low porosity tubes (40) which are suspended in the reactor chamber (39) and which substantially traverse the matrix; expended medium and cell product is withdrawn through relatively high porosity tubes (41) which are also suspended in the reactor chamber (39) and which substantially traverse the matrix; and oxygenated gaseous medium is supplied by perfusion into the matrix through a selectively-permeable membrane (42) disposed in the reactor chamber (39). |
申请公布号 |
DE3377800(D1) |
申请公布日期 |
1988.09.29 |
申请号 |
DE19833377800 |
申请日期 |
1983.12.06 |
申请人 |
MONSANTO COMPANY |
发明人 |
FEDER, JOSEPH;LEWIS, CHARLES, JR.;TOLBERT, WILLIAM ROBERT |
分类号 |
C12N15/02;C12M3/00;C12M3/06;C12N5/00;C12N5/06;C12N15/00;C12P21/08;C12R1/91;(IPC1-7):C12M3/02 |
主分类号 |
C12N15/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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