摘要 |
PURPOSE:To increase the quantity of the fluorescence to be received from respective measurement points by irradiating the measuring parts on respective migration paths to a linear shape with the same laser light to correct the change in the quantity of the fluorescence by the fluctuation of the laser and using a two-dimensional detector. CONSTITUTION:The laser light from laser light sources 4, 4' irradiate the gel to the linear shape alternately from the lower side face of an electrophoresis plate 1. Samples A, B,... are injected from a buffer chamber 3 and migrate downward. The samples emit the fluorescence at the time of passing the light projection part. The images formed by the light emission are condensed by an imaging lens 5 and are spectrally split by a diffraction grating 6 so that the images are formed on an image amplifier 7. The light beams of different wavelengths are dispersed above and below and appear in the positions deviated according to colors on the two-dimensional detector 9 via a coupling lens 8. The ways of the appearance are visible with the images on a monitor 13. The vertical direction is the wavelength dispersion and the fluorescence can be observed as a broad band. The horizontal direction is the samples A, B,.... |