| 摘要 |
PURPOSE:To separate and analyze an enantiomeric mixture of chrysanthemumic acid esters with an excellent separating efficiency in a short time, by analyzing a specific enantiomeric mixture of chrysanthemumic acid esters, which is a pyrethroid insecticide, by liquid chromatography using a stationary phase wherein an optically active N-acylated amino acid is grafted. CONSTITUTION:As a packing material having a grafted N-acylated amino acid, e.g., a chromatographic packing material wherein an optically active organosilane represented by formula I is grafted onto an inorganic carrier having a hydroxyl group on its surface, is used as a stationary phase. An enantiomeric mixture of chrysanthemumic acid esters represented by formula II is separated by liquid chromatography. In the formulas, R is a lower alkyl group or a halogen atom; A is an alkyl group including optionally substituted aryl, aralkyl, cycloalkyl or heterocyclic group; R1-R3 are each an alkyl group, an alkoxy group, a hydroxyl group, or a halogen atom; R4 is a lower alkyl or phenyl group; (n) is 2-4; and X is an -NHCO- group or the like. |
