ASSAY OF Y-SPERMATOZOON OF MAMMAL

摘要

PURPOSE:To permit assay of Y-spermatozoa by previously cleaning the spermatozoa of a mammal with a phosphate buffer soln. and treating the same with protease, dyeing the Y-spermatozoa (the spermatozoa having Y chromosomes) of such spermatozoa with quinacrine mustard to that the F-bodies (small bodies which emit fluorescence) can be visually observed. CONSTITUTION:The Y-spermatozoa of the spermatozoa of the mammal which are previously cleaned by the phosphate buffer soln. such as Ringer's soln. and are treated by the protease such as 'Dispase(R)' are dyed by the quinacrine mustard. The dyeing by the quinacrine mastered is executed by using a quinacrine mustard soln. prepd. by dissolving the quinacrine mustard at about 0.0025-0.0050% in the phosphate buffer soln. The dyed F-bodies can be observed by inspecting the dyed spermatozoa with a fluorescent microscope. The number of the Y-spermatozoa is known by counting the number of the dyed F-bodies.