METHOD AND DEVICE FOR THE SOLID-PHASE SEQUENCING OF NUCLEIC-ACID FRAGMENTS

摘要

A device for solid phase sequencing of nucleic acid segments comprises a plurality of sequencing blocks for storaging and processing n nucleic acid fragments immobilized on carrier segments or in homogenous phase during a sequencing process, each of a sample dosing device for immobilizing n labeled nucleic acid samples from the sequencing blocks onto areas of a carrier matrix in a simultaneous fashion and including a capillary holder and a plurality of capillaries corresponding to the number n of different nucleic acid samples to be sequences and arranged according to the distribution of the reaction vessels with a distance between capillary orifices from the capillary holder greater than a depth of the reaction vessels, a plurality of carrier holders for fixing carrier matrices in a predetermined position during the immobilization of the nucleic acid samples with the sample dosing device and each having a recess corresponding to respective dimensions of the carrier matrix and a plurality of bores with a number and distribution pattern corresponding to those of the reaction vessels and with a diameter which is somewhat larger than a diameter of the capillary, a punch for simultaneous punching of n immobilized and chemically modified nucleic acid samples as individual carrier segments out of the carrier matrix into the sequencing blocks and including a punching plate and a plurality of punching pins with a number and distribution pattern which also corresponds to those of the reaction vessels.