Method for the photometric determination of biological agglutination
摘要
A photometric method and apparatus for measuring agglutination in a biological agglutination reaction system test sample using a laser beam source and at least one photodetector for detecting light scattered by the test sample. The method includes the following steps: (1) arranging the at least one photodetector so as to be capable of detecting scattered light from the test sample at a scatter angle of 30 to 60 with respect to a laser beam directed at the test sample from the laser beam source; (2) irradiating the test sample with the laser beam from the laser beam source; (3) selectively detecting the intensity of scattered light from the test sample at the scatter angle of 30 to 60 using the at least one photodetector which provides an output indicative thereof; and (4) determining the first derivative of the output of the at least one photodetector with respect to time and obtaining the maximum value thereof. In addition to the laser beam source and the at least one photodetector, the apparatus also includes a reaction cuvette for containing the test sample.