| 摘要 |
A cassette vector system contains the following system: a) a segment from an E. coli plasmid having a replication origin and selection marker effective in E. coli, b) replaceable segments effective in higher eukaryotic cells, namely b<1>) a promoter, b<2>) a translation initiator, b<3>) a polylinker, b<4>) a splicing signal and b<5>) a polyadenylation signal, where two of these replaceable segments can also be arranged as jointly replaceable units, characterised by the DNA sequences (coding strand> ATG GAT AGA TCT (1> ATG GAT AGA TCT C (2> ATG GAT AGA TCT CC (3> where the ATG triplet at the 5'-end of the sequences (1), (2) and (3) is the only translation start codon which is arranged functionally behind the promoter, and where the polylinker b<3>) follows the sequences (1), (2) and (3). <IMAGE> |
