| 摘要 |
PURPOSE:To obtain the titled alcohol and an optically active ester which is an unreacted substance useful as a raw material for medicines, agricultural chemicals or liquid crystals, etc., by reacting a racemic ester with a enzyme derived from a microorganism. CONSTITUTION:Racemic modification of a substrate expressed by formula I (R1 is 1-14C aliphatic hydrocarbon group; R2 is 1-8C aliphatic hydrocarbon group; X is halogen) is suspended in a reaction solution in a concentration within the range of 2-60% and an enzyme, derived from a microorganism for the genus Pseudomonas, Chromobacterium, Aspergillus or Candida and having stereoselective esterase activity is added so as to provide 1:1-1:1,000 weight ratio of the enzyme to the substrate. Reaction is carried out at 10-45 deg.C, preferably 30-40 deg.C and pH 4.0-8.5, preferably pH 6.0-7.5 while adjusting the pH to afford the aimed alcohol, expressed by formula II and having optical activity and optically active ester expressed by formula III. The ester expressed by formula III can be converted into the alcohol expressed by formula II by refluxing in methanol for several hr. |
