| 摘要 |
Antibodies (Ab) specific for toxins of Clostridium difficile, opt. bound to a solid support, are new. Esp. Ab are monospecific for either A or B toxin. Also new are assays for toxigenic C. difficile using Ab. The immobilised Ab is incubated with the test sample to bind C. difficile antigens. The bound antigen is then incubated with labelled, monospecific Ab, and the presence of label determined by detecting presence of the label. Particularly, the label used is alkaline phosphatase. In a modification, the monospecific Ab is not labelled, and detection is by reaction with a second, labelled antibody specific for the first. Alternatively, toxins are detected by agglutination of appropriately sensitised solid particles (or by inhibition of such agglutination). |
