AUTORADIOGRAPHIC GENE-SCREENING METHOD

摘要

An autoradiographic gene-screening method employing a hybridization process, which comprises: (1) a step of transferring at least a portion of nucleic acids, fragments thereof or derivatives thereof resolved on a medium onto a transfer support to fix them thereonto; (2) a step of hybridizing the nucleic acids, fragments thereof or derivatives thereof fixed onto said transfer support with radioactively labeled probes; and (3) a step of obtaining locational information on the radioactively labeled substances on said transfer support, which comprises placing said transfer support having been subjected to the hybridization and a stimulable phosphor sheet in layers for a given period of time to cause said sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on said transfer support, exciting said stimulable phosphor sheet with an electromagnetic wave to release the radiation energy stored in said sheet as stimulated emission, and detecting the stimulated emission.