| 摘要 |
PURPOSE:To shorten the time for operation and to decrease the amt. of a sample by disposing, in series, solid phase parts where antibodies corresponding to plural kinds of the antigens to be measured are immobilized to respectively different solid phase parts into a flow passage. CONSTITUTION:A buffer soln. 5 is kept passing continuously by using a liquid feed pump 4 to reaction columns 1-3 which are connected in series and in which the antibodies of different kinds are immobilized. The sample liquid to be measured is introduced therein by using a cut valve 6. After the sample passes the columns 1-3, the sample is discharged from a flow passage 8 via a 3-way cock 7 and is poured into a liquid discharge reservoir 9. The antibodies labeled with glucose oxidase are then prepared for the respective kinds of the antibodies and are mixed to attain approximately the same concn. Such liquid mixture is injected and passed through the columns 1-3 like the sample and is then poured through the cock 7 into the reservoir 9. The columns are cleaned after the injection of the liquid mixture therein and the conjugation amt. of the labeling antibodies is electrochemically detected. Cocks 10-13 are operated to bypass 14, 15 the liquid so as not to pass the columns 2, 3 in order to make measurement in the column 1, thereby executing the detection 16.
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