A NOVEL IMMUNOAFFINITY PURIFICATION SYSTEM

摘要

A stable activated affinity support having single aldehyde functionalities at the termini of extended spacer arms. The support allows covalent attachment of proteins and ligands under physiological conditions with a maximal retention of biological activity. This is examplified by the covalent immobilization of pepsin, avidin, Protein A and immunoglobulin G. The activated adsorbent is also prepared in a magnetically-responsive form. An elution buffer for recovering antigen or antibody from immunoadsorbents is described and an immunoaffinity purification system is established for quantitative recovery of antigens from immunoadsorbents while retaining their biological acivity.