发明名称 |
Modulation of gene expression through interference with RNA secondary structure |
摘要 |
Methods for modulating the expression of viral genes are provided by selecting a portion of RNA coded by the gene, said RNA portion having subportions forming a secondary structure, and contacting the RNA with oligonucleotide of 6 to 50 which can bind with at least one of said subportions of the RNA. In accordance with the preferred embodiments, oligonucleotides are designed to bind to RNA secondary structures which are of significance to the expression of the gene coding for said RNA. In accordance with a preferred embodiment, methods of treatment of human immunodeficiency virus are similarly disclosed wherein the oligonucleotides are targeted at the CAR or gag-pol region of HIV RNA.
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申请公布号 |
US5866698(A) |
申请公布日期 |
1999.02.02 |
申请号 |
US19940227180 |
申请日期 |
1994.04.13 |
申请人 |
ISIS PHARMACEUTICALS, INC. |
发明人 |
ECKER, DAVID;VICKERS, TIMOTHY A.;BRUICE, THOMAS W. |
分类号 |
C12N15/113;C12N15/63;C12N15/67;(IPC1-7):C07H21/02;C07H21/04 |
主分类号 |
C12N15/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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