METHOD FOR THE REMOVAL OF UNDESIRED CELLS FROM HUMAN LYMPHOCYTE POPULATIONS, APPLICATION OF THE METHOD IN MONOCLONAL ANTIBODY PRODUCTION AND KIT THEREFOR

摘要

A method for the removal of, for in vitro immunisation, undesired cells from human lymphocyte containing cell populations. For the method, use is made of lysosomotropic agents which are caused to act in vitro on human lymphocyte containing cell populations. Also disclosed is a method in the in vitro immunisation of lymphocytes for use in the production of human monoclonal antibodies. In this method, lysosomotropic agents are caused to act in vitro on human lymphocyte containing cell populations for removing cell populations having a negative effect on the in vitro immunisation, whereupon the lymphocytes are in vitro immunised antigen-specifically and immortalised. Finally, a kit for in vitro immunisation of lymphocytes for use in the production of human monoclonal antibodies is disclosed. The kit comprises as active constituents a container containing lymphokines and a container containing lysosomotropic agents.