发明名称 |
Method of producing reverse transcriptase |
摘要 |
A method of producing reverse transcriptase, which comprises isolating a fraction containing retrovirus from a tissue culture fluid supernatant of retrovirus producing cells which are able to grow and propagate in vitro, treating said fraction at least once by sucrose density gradient centrifugation to thereby obtain a purified retrovirus, and extracting reverse transcriptase from said purified retrovirus.
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申请公布号 |
US4729955(A) |
申请公布日期 |
1988.03.08 |
申请号 |
US19830559383 |
申请日期 |
1983.12.08 |
申请人 |
NAT INST ANIMAL HEALTH |
发明人 |
KODAMA, MICHI;SEKIGUCHI, KIICHI;KUBO, MASANORI |
分类号 |
C12N5/06;C12N9/12;C12N15/09;C12R1/91;(IPC1-7):C12N9/12 |
主分类号 |
C12N5/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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