Generation of a cancer-specific immune response toward MUC1 and cancer specific MUC1 antibodies

摘要

The present invention provides a method for inducing a cancer specific immune response against MUC1 using an immunogenic glycopeptide. Other aspects of the invention are a pharmaceutical composition comprising the immunogenic glycopeptide and a cancer vaccine comprising the immunogenic glycopeptide. Another aspect is an antibody generated using the immunogenic glycopeptide and the use of said antibody in therapy and diagnosis.

主权项

1. A method for the preparation of a monoclonal antibody comprising:
selecting against a peptide library generated by a molecular display technique with an immunogenic glycopeptide comprising a GSTA motif, wherein the GSTA motif is present in a tandem repeat, wherein said GSTA motif is O-glycosylated at least at the T-residue or at the S-residue of the GSTA motif wherein the sequence of the tandem repeat is VTSAPDTRPAPGSTAPPAHG (SEQ ID NO. 1), where in the glycosylation pattern of the tandem repeat is selected from the group consisting of: VTTnSAPDTRPAPGSTTnAPPAHG VTTnSAPDTRPAPGSTnTTnAPPAHG VTTnSAPDTTnRPAPGSTnTTnAPPAHG VTTnSTnAPDTRPAPGSTnTTnAPPAHG VTTnSTnAPDTTnRPAPGSTnTTnAPPAHG VTSTnSAPDTRPAPGSTSTnAPPAHG VTSTnSAPDTRPAPGSSTnTSTnAPPAHG VTSTnSAPDTSTnRPAPGSSTnTSTnAPPAHG VTSTnSSTnAPDTRPAPGSSTnTSTnAPPAHG VTSTnSSTnAPDTSTnRPAPGSSTnTSTnAPPAHG; and
preparing a monoclonal antibody therefrom, which specifically binds said immunogenic glycopeptide comprising a GSTA motif, wherein said GSTA motif is O-glycosylated at least at the T-residue or at the S-residue of the GSTA motif.