Human monoclonal antibody with specificity for Dengue virus serotype 1 E protein and uses thereof

摘要

Compositions and methods for the treatment or prevention of Dengue virus infection in a vertebrate subject are provided. In particular, human neutralizing monoclonal antibodies to Dengue virus isolated from EBV immortalized B cells derived from patients who have recovered from Dengue infection are disclosed. Methods are provided for administering such antibodies to a vertebrate subject in an amount effective to reduce, eliminate, or prevent relapse from infection.

主权项

1. An isolated neutralizing monoclonal antibody or fragment thereof that specifically binds to a Dengue virus serotype 1 envelope (E) protein or fragment thereof, wherein the antibody is a human antibody with broadly neutralizing activity that binds across two E proteins in a virus, wherein the binding across two E proteins comprises binding to DI and the hinge between DI and II on one E protein and DIII of a neighboring E protein, and wherein the antibody or fragment thereof binds to amino acids T51, G159, T160, T165, P166, Q167, E172, I173, and T275 of SEQ ID NO:33 in DI, amino acids N52 and G274 of SEQ ID NO:33 in DII, and K310 of SEQ ID NO:33 in DIII.