摘要 |
Previously unknown hybrid α-amylases of the formula <IMAGE> where Q is an N-terminal polypeptide residue having from 55 to 60 amino acid residues, at least 75% of which are homologous with the 55 N-terminal amino acid residues in Bacillus amyloliquefaciens α-amylase as described in Takkinen et al., J.Biol.Chem. 258 (1983) 1007 - 1013, R is a polypeptide residue of the general formula Pro-Tyr-Asp-Leu-Tyr-Asp-Leu-Gly-Glu-Phe-X8-Gln-Lys- Gly-Thr-Val-Arg-Thr-Lys-Tyr-Gly-Thr-Lys-X9-Glu-Leu- Gln-X10-Ala-Ile-Lys where X8 is His or Gln, X9 is Gly or Ser and X10 is Ser or Asp, and L is a C-terminal polypeptide of from 390 to 400 amino acid residues, at least 75 per cent of which are homologous with the 395 C-terminal amino acid residues in Bacillus licheniformis 584 (ATCC 27811) α-amylase. <IMAGE> |