发明名称 |
Inverted latency specific binding assay |
摘要 |
A specific binding assay composition and method for determining a ligand in a sample are disclosed. The composition comprises (a) a binding partner for the ligand; (b) a detection system which has at least two components; (c) a selectively accessible vesicle having a surface-incorporated ligand or ligand analog and a first component of the detection system therein; (d) a substance which modifies vesicle accessibility in response to binding of surface-associated ligand or ligand analog and binding partner; and (e) at least one additional component of the detection system which is reactive with the first component to produce a detectable response which is reduced by association of the binding partner and vesicle modifying substance with the variabily accessible vesicle. A decrease in signal is measured upon reaction as compared to the signal of unreacted vesicles.
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申请公布号 |
US4713324(A) |
申请公布日期 |
1987.12.15 |
申请号 |
US19830528496 |
申请日期 |
1983.09.01 |
申请人 |
TECHNICON INSTRUMENTS CORPORATION |
发明人 |
FOX, JOHN P.;HEDAYA, EDDIE;LIPPMAN, VIOLET |
分类号 |
G01N33/544;A61K39/00;C12Q1/00;C12Q1/26;C12Q1/28;G01N33/53;G01N33/542;G01N33/543;G01N33/554;(IPC1-7):C12Q1/00 |
主分类号 |
G01N33/544 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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