| 摘要 |
<p>A process for recovering alpha -L-aspartyl-L-phenylalanine-methyl ester ( alpha -APM) or the hydrochloride thereof from a solution mixture containing (I) alpha -L-aspartyl-L-phenylalanine dimethyl ester ( alpha -A(M)PM), and the corresponding demethylation derivatives (II), i.e. alpha -L-aspartyl-L-phenylalanine methyl ester ( alpha -APM), alpha -L-aspartyl methyl ester-L-phenylalanine, wherein the methyl ester group is bound to the beta -carboxy group of the aspartyl residue ( alpha -A(M)P), and alpha -L-aspartyl-L-phenylalanine ( alpha -AP), and at least one inorganic chloride, comprises desalting and concentrating a solution mixture in which the weight ratio of (I) to (II) is not greater than 1.0 and the ratio of the inorganic chloride content (III) to the sum of the compounds (I+II), i.e. III/(I+II) in terms of the weight ratio is not less than 1.0, by means of a reverse osmosis membrane having a NaCl inhibition rate of 30 to 80 % until said ratio of the inorganic chloride content to the content of compounds I and II, i.e. III/(I+II) attains a value in the range of 0.3 to 1.3 in terms of the weight ratio, then bringing the concentrate into contact with an aqueous solvent containing methanol and hydrochloric acid to precipitate alpha -L-aspartyl-L-phenylalanine methyl ester ( alpha -APM) hydrochloride and optionally neutralizing the hydrochloride.</p> |
