| 摘要 |
The novel L-sorbose-dehydrogenase according to the invention catalyses the oxidation of L-sorbose to L-sorboson, the precursor of 2-keto-L-gulonic acid which, in turn, is an important intermediate in the preparation of vitamin C. …<??>The process according to the invention for the preparation of the novel L-sorbose-dehydrogenase is carried out by culturing a microorganism belonging to the genus Gluconobacter, or a mutant of this microorganism, which is capable of producing the novel L-sorbose- dehydrogenase in the cell material, breaking up the cell material, isolating the L-sorbose-dehydrogenase from cell-free extract of the broken-up cell material, preferably from the membrane fraction of the cell material, and purifying the L-sorbose-dehydrogenase. |
