| 摘要 |
PURPOSE:To sustain the nitrile hydration activity of an enzyme nitrilase produced by a microorganism, by adding at least one of nitriles, amides and organic acids or salts thereof as a stabilizer to a solution of the nitrilase or an immobilized material thereof. CONSTITUTION:A strain, etc., of the genuses Corynebacterium, Nocardia, Rhodococcus, Bacillus, Bacteridium, Micrococcus and Brevibacterium is cultivated in a nutrient culture medium under cultivation condition of 5-9pH, preferably about 6-8pH and 20-37 deg.C, preferably 20-30 deg.C for 1-3 days to give nitrilase. At least one of nitriles, amides and organic acids and salts thereof, in normally 0.01-50g/l, preferably 0.1-10g/l concentration as a stabilizer is added to a solution or suspension of the above-mentioned enzyme and an immobilized material thereof to stably sustain the nitrile hydration activity for a long period.
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