Analytical method for detecting and measuring specifically sequenced nucleic acid.

摘要

A waveguide coated with single-stranded probe nucleic acids and carrying an internally reflected wave signal is contacted with an analyte solution containing denatured test DNA or RNA and fluorescent marker dye. Analyte nucleic acid with sequences homologous to that of the probe polynucleotide will hybridize therewith with concomitant binding of the fluorescent dye to the resultant duplex structures. Fluorescence resulting from the interaction of the wave signal at the waveguide/analyte interface with the signal generating centers created within the space probed by the evanescent component of the wave signal is detected and provides useful information on said sequences homologous to that of the probe nucleic acids.